Issue 97, 2013
From the journal:

Chemical Communications

Flagella as a novel alignment medium for the measurement of residual dipolar couplings in proteins†

Himanshu Singh,a   Manish Shukla,ab   Basuthkar J. Raoa  and  Kandala V. R. Chary*ab  

* Corresponding authors

a Tata Institute of Fundamental Research, Mumbai, India
E-mail: chary@tifr.res.in
Web: http://www.tifr.res.in/∼chary/
Fax: +91 22 2280-4610
Tel: +91 22 2278-2489

b Tata Institute of Fundamental Research, Center for Interdisciplinary Sciences, Hyderabad, India

Abstract

The two flexible rod-like flagella (∼500 nm in diameter and 5–15 μm long) of Chlamydomonas reinhardtii, a unicellular green alga, can weakly align molecules in an external magnetic field, thereby enabling the measurement of various residual dipolar couplings in solution NMR spectroscopy.

Graphical abstract: Flagella as a novel alignment medium for the measurement of residual dipolar couplings in proteins

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Article information

DOI
https://doi.org/10.1039/C3CC46233A
Article type
Communication
Submitted
14 Aug 2013
Accepted
10 Oct 2013
First published
10 Oct 2013

Chem. Commun., 2013,49, 11403-11405

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Flagella as a novel alignment medium for the measurement of residual dipolar couplings in proteins

H. Singh, M. Shukla, B. J. Rao and K. V. R. Chary, Chem. Commun., 2013, 49, 11403 DOI: 10.1039/C3CC46233A

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