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Issue 84, 2013
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Detection of salt bridges to lysines in solution in barnase

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Abstract

We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.

Graphical abstract: Detection of salt bridges to lysines in solution in barnase

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Article information


Submitted
23 Jul 2013
Accepted
05 Sep 2013
First published
05 Sep 2013

Chem. Commun., 2013,49, 9824-9826
Article type
Communication

Detection of salt bridges to lysines in solution in barnase

M. P. Williamson, A. M. Hounslow, J. Ford, K. Fowler, M. Hebditch and P. E. Hansen, Chem. Commun., 2013, 49, 9824
DOI: 10.1039/C3CC45602A

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