Issue 67, 2013
From the journal:

Chemical Communications

Translational repression using BIV Tat peptide–TAR RNA interaction in mammalian cells†

Chaitanya Sudrik,a   Manish Arha,b   Jicong Cao,a   David V. Schaffer*cde  and  Ravi S. Kane*ab  

* Corresponding authors

a Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
E-mail: kaner@rpi.edu

b Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA

c Department of Chemical Engineering, University of California Berkeley, Berkeley, California, USA
E-mail: schaffer@berkeley.edu

d Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, California, USA

e California Institute for Quantitative Biosciences, University of California Berkeley, Berkeley, California, USA

Abstract

We developed a strategy to create novel genetically encoded switches based on translational repression. We illustrated its efficacy by incorporating two copies of an RNA hairpin in the 5′-untranslated region (UTR) of a target mRNA and demonstrating 7-fold translational repression upon expression of a ligand – the BIV Tat peptide.

Graphical abstract: Translational repression using BIV Tat peptide–TAR RNA interaction in mammalian cells

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Article information

DOI
https://doi.org/10.1039/C3CC43086C
Article type
Communication
Submitted
25 Apr 2013
Accepted
05 Jul 2013
First published
15 Jul 2013

Chem. Commun., 2013,49, 7457-7459

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Translational repression using BIV Tat peptide–TAR RNA interaction in mammalian cells

C. Sudrik, M. Arha, J. Cao, D. V. Schaffer and R. S. Kane, Chem. Commun., 2013, 49, 7457 DOI: 10.1039/C3CC43086C

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