Issue 55, 2013
From the journal:

Chemical Communications

ELISA detection of semicarbazide based on a fast sample pretreatment method†

Zhiyuan Fang,‡a   Baishan Jiang,‡a   Wei Wu,a   Zhicheng Xiang,a   Chuanyan Ouyang,a   Tulong Huang,a   Junhua Chena  and  Lingwen Zeng*a  

* Corresponding authors

a Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
E-mail: zeng6@yahoo.com
Fax: +86 20 32015245
Tel: +86 20 32015312

Abstract

A direct ELISA was established for the fast detection of semicarbazide (SEM) using a novel biotin derivative. Without a tedious extraction procedure, as low as 0.07 μg L−1 of SEM could be detected reproducibly. This assay has better recovery and accuracy than competitive ELISA.

Graphical abstract: ELISA detection of semicarbazide based on a fast sample pretreatment method

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Article information

DOI
https://doi.org/10.1039/C3CC42790K
Article type
Communication
Submitted
16 Apr 2013
Accepted
23 May 2013
First published
23 May 2013

Chem. Commun., 2013,49, 6164-6166

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ELISA detection of semicarbazide based on a fast sample pretreatment method

Z. Fang, B. Jiang, W. Wu, Z. Xiang, C. Ouyang, T. Huang, J. Chen and L. Zeng, Chem. Commun., 2013, 49, 6164 DOI: 10.1039/C3CC42790K

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