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Issue 47, 2013
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A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells

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Abstract

A ratiometric EuIII complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pKa of 7.15.

Graphical abstract: A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells

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Publication details

The article was received on 29 Mar 2013, accepted on 30 Apr 2013 and first published on 01 May 2013


Article type: Communication
DOI: 10.1039/C3CC42308E
Citation: Chem. Commun., 2013,49, 5363-5365

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    A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells

    B. K. McMahon, R. Pal and D. Parker, Chem. Commun., 2013, 49, 5363
    DOI: 10.1039/C3CC42308E

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