A technique for measuring distances between two or more fluorophores spaced in the 10–100 nm range is described. We identify a linear correlation between the intensity–amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.
R. L. Boulineau and M. A. Osborne, Chem. Commun., 2013, 49, 5559 DOI: 10.1039/C3CC42072H
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