Issue 37, 2013
From the journal:

Chemical Communications

Directed evolution of a synthetic RNA–protein module to create a new translational switch†

Tomoaki Hara,a   Hirohide Saito*bcd  and  Tan Inoue*ab  

* Corresponding authors

a Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto, Japan

b International Cooperative Research Project (ICORP), Japan Science and Technology Agency (JST), 5 Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan
E-mail: tan@kuchem.kyoto-u.ac.jp
Fax: +81 75 366 7096
Tel: +81 75 366 7029

c The Hakubi Center for Advanced Research, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto, Japan

d Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan
E-mail: hirohide.saito@cira.kyoto-u.ac.jp

Abstract

A synthetic RNP-binding module was developed to produce an alternative translational switch: an in vitro selection experiment targeting a derivative of L7Ae protein (L7KK) identified a new H23 RNA aptamer that binds tightly to both L7KK and L7Ae. The switch serves as a translational OFF switch for constructing a NOR gate.

Graphical abstract: Directed evolution of a synthetic RNA–protein module to create a new translational switch

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Article information

DOI
https://doi.org/10.1039/C3CC38688K
Article type
Communication
Submitted
04 Dec 2012
Accepted
17 Jan 2013
First published
21 Jan 2013

Chem. Commun., 2013,49, 3833-3835

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Directed evolution of a synthetic RNA–protein module to create a new translational switch

T. Hara, H. Saito and T. Inoue, Chem. Commun., 2013, 49, 3833 DOI: 10.1039/C3CC38688K

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