Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate

Katerina Busuttil; Alexandru Rotaru; Mingdong Dong; Flemming Besenbacher; Kurt V. Gothelf

doi:10.1039/C3CC37408D

Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate

Katerina Busuttil,^a Alexandru Rotaru,^a^b Mingdong Dong,^a Flemming Besenbacher^a and Kurt V. Gothelf*^a^c

* Corresponding authors

^a Danish National Research Foundation: Centre for DNA Nanotechnology (CDNA) at Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Langelandsgade 140, DK-8000 Aarhus C, Denmark
E-mail: kvg@chem.au.dk
Fax: +45 8619 6199
Tel: +45 8715 5952

^b Center of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry, Aleea Grigore Ghica Voda 41A, 700487 Iasi, Romania

^c Department of Chemistry, Aarhus University, Langelandsgade 140, DK-8000 Aarhus C, Denmark

Abstract

Patterns of the protein streptavidin on DNA origami are printed onto a functionalized surface. The streptavidins are linked to DNA via a disulfide spacer and these conjugates self-organise into a predetermined pattern on DNA origami. The structure immobilises on a biotin-SAM-functionalized Au surface. By disulfide cleavage the origami is separated from the streptavidins, leaving the protein pattern at the surface.

This article is part of the themed collection: Nucleic acids: new life, new materials

Supplementary files

Publication details

The article was received on 10 Oct 2012, accepted on 10 Jan 2013 and first published on 10 Jan 2013

Article type: Communication

DOI: 10.1039/C3CC37408D

Citation: Chem. Commun., 2013,49, 1927-1929

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Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate

K. Busuttil, A. Rotaru, M. Dong, F. Besenbacher and K. V. Gothelf, Chem. Commun., 2013, 49, 1927
DOI: 10.1039/C3CC37408D
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Chemical Communications

Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate

Abstract

Supplementary files

Publication details

Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate

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