Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 36, 2013
Previous Article Next Article

Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

Author affiliations

Abstract

Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.

Graphical abstract: Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Jan 2013, accepted on 18 Mar 2013 and first published on 18 Mar 2013


Article type: Communication
DOI: 10.1039/C3CC00070B
Citation: Chem. Commun., 2013,49, 3754-3756

  •   Request permissions

    Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

    Y. J. Yeo, K. Roh, J. Y. Bang, E. H. Lee, H. S. Park and D. Kim, Chem. Commun., 2013, 49, 3754
    DOI: 10.1039/C3CC00070B

Search articles by author

Spotlight

Advertisements