Issue 36, 2013
From the journal:

Chemical Communications

Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis†

Youn Jee Yeo,‡a   Kyoungmin Roh,‡b   Joo Young Bang,a   Eun Hee Lee,a   Hyung Soon Park*a  and  Dong-Eun Kim*b  

* Corresponding authors

a R&D Center, Diatech Korea Co. Ltd., Seoul 138-200, Republic of Korea

b Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea
E-mail: kimde@konkuk.ac.kr, hspark@diatech.co.kr
Fax: +82-2-3436-6062
Tel: +82-2-2049-6062

Abstract

Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.

Graphical abstract: Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C3CC00070B
Article type
Communication
Submitted
04 Jan 2013
Accepted
18 Mar 2013
First published
18 Mar 2013

Chem. Commun., 2013,49, 3754-3756

Permissions

Request permissions

Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

Y. J. Yeo, K. Roh, J. Y. Bang, E. H. Lee, H. S. Park and D. Kim, Chem. Commun., 2013, 49, 3754 DOI: 10.1039/C3CC00070B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements