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Issue 15, 2013
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Label-free electrochemical detection of an Entamoeba histolyticaantigen using cell-free yeast-scFv probes

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Abstract

Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.

Graphical abstract: Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes

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Submitted
12 Dec 2012
Accepted
07 Jan 2013
First published
07 Jan 2013

Chem. Commun., 2013,49, 1551-1553
Article type
Communication

Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes

Y. S. Grewal, M. J. A. Shiddiky, S. A. Gray, K. M. Weigel, G. A. Cangelosi and M. Trau, Chem. Commun., 2013, 49, 1551
DOI: 10.1039/C2CC38882K

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