Issue 24, 2013
From the journal:

Chemical Communications

Layer by layer assembly of biotinylated protein networks for signal amplification†

Yu W. Chu,a   Bo Y. Wang,a   Huei-Shian Lin,a   Tai-Yen Lin,a   Yuan-Jen Hung,a   David A. Engebretson,b   Wonjae Lee*c  and  James R. Carey*a  

* Corresponding authors

a Department of Applied Chemistry, National University of Kaohsiung, 700 Kaohsiung University Road, Kaohsiung 811, Taiwan
E-mail: jcarey@nuk.edu.tw
Fax: +886-7-591-9348
Tel: +886-7-591-9778

b Department of Chemistry, Oklahoma City University, Oklahoma City, OK, USA

c College of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea
E-mail: wlee@chosun.ac.kr
Fax: +82-62-222-5414
Tel: +82-62-230-6376

Abstract

Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin–biotin recognition event. Amplification induced by streptavidin and biotinylated protein causes the formation of a large detectable polymer. This approach enjoys a 100-fold decrease in detection limit in comparison with the commercial methods.

Graphical abstract: Layer by layer assembly of biotinylated protein networks for signal amplification

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Article information

DOI
https://doi.org/10.1039/C2CC38233D
Article type
Communication
Submitted
15 Nov 2012
Accepted
07 Jan 2013
First published
07 Jan 2013

Chem. Commun., 2013,49, 2397-2399

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Layer by layer assembly of biotinylated protein networks for signal amplification

Y. W. Chu, B. Y. Wang, H. Lin, T. Lin, Y. Hung, D. A. Engebretson, W. Lee and J. R. Carey, Chem. Commun., 2013, 49, 2397 DOI: 10.1039/C2CC38233D

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