Jump to main content
Jump to site search

Issue 10, 2013
Previous Article Next Article

Controllably degradable β-sheet nanofibers and gels from self-assembling depsipeptides

Author affiliations

Abstract

Self-assembled peptide materials have received considerable interest for a range of applications, including 3D cell culture, tissue engineering, and the delivery of cells and drugs. One challenge in applying such materials within these areas has been the extreme stability of β-sheet fibrillized peptides, which are resistant to proteolysis, degradation, and turnover in biological environments. In this study, we designed self-assembling depsipeptides containing ester bonds within the peptide backbone. Beta-sheet fibrillized nanofibers were formed in physiologic conditions, and two of these nanofiber-forming depsipeptides produced hydrogels that degraded controllably over the course of days-to-weeks via ester hydrolysis. With HPLC, TEM, and oscillating rheometry, we show that the rate of hydrolysis can be controlled in a straightforward manner by specifying the amino acid residues surrounding the ester bond. In 3D cell cultures, depsipeptide gels softened over the course of several days and permitted considerably more proliferation and spreading of C3H10T1/2 pluripotent stem cells than non-degradable analogs. This approach now provides a reliable and reproducible means to soften or clear β-sheet fibrillized peptide materials from biological environments.

Graphical abstract: Controllably degradable β-sheet nanofibers and gels from self-assembling depsipeptides

Back to tab navigation

Supplementary files

Publication details

The article was received on 28 Jun 2013, accepted on 25 Jul 2013 and first published on 13 Aug 2013


Article type: Paper
DOI: 10.1039/C3BM60161G
Citation: Biomater. Sci., 2013,1, 1037-1045

  •   Request permissions

    Controllably degradable β-sheet nanofibers and gels from self-assembling depsipeptides

    Y. F. Tian, G. A. Hudalla, H. Han and J. H. Collier, Biomater. Sci., 2013, 1, 1037
    DOI: 10.1039/C3BM60161G

Search articles by author

Spotlight

Advertisements