Jump to main content
Jump to site search

Issue 20, 2013
Previous Article Next Article

Development of poly((3-acrylamidophenyl)boronic acid-co-N,N-methylenebisacrylamide) monolithic capillary for the selective capture of cis-diol biomolecules

Author affiliations

Abstract

A new hydrophilic boronate affinity monolithic capillary was synthesized for the selective capture of cis-diol biomolecules including glycoproteins and nucleosides, using (3-acrylamidophenyl)boronic acid (APBA) and N,N-methylenebisacrylamide (MBAA) as the functional monomer and cross-linker, respectively. A comparison with other compositionally similar monolithic capillaries was carried out in terms of reversed-phase retention, selectivity, binding capacity and binding pH. The poly(APBA-co-MBAA) monolithic capillary exhibited notably different properties. As both the functional monomer and the cross-linker are hydrophilic, the monolith capillary showed suppressed reversed-phase retention and as a result excellent selectivity toward both nucleosides and glycoproteins was observed. It exhibited the highest binding capacity as compared with other compositionally similar monolithic capillaries. Besides, because of the lower pKa value of APBA, the monolithic capillary was able to selectively capture nucleosides at near-physiological pH.

Graphical abstract: Development of poly((3-acrylamidophenyl)boronic acid-co-N,N-methylenebisacrylamide) monolithic capillary for the selective capture of cis-diol biomolecules

Back to tab navigation

Publication details

The article was received on 25 Jun 2013, accepted on 16 Aug 2013 and first published on 19 Aug 2013


Article type: Paper
DOI: 10.1039/C3AY41045E
Citation: Anal. Methods, 2013,5, 5444-5449
  •   Request permissions

    Development of poly((3-acrylamidophenyl)boronic acid-co-N,N-methylenebisacrylamide) monolithic capillary for the selective capture of cis-diol biomolecules

    X. Wang, Y. Liu, L. Ren, H. Li and Z. Liu, Anal. Methods, 2013, 5, 5444
    DOI: 10.1039/C3AY41045E

Search articles by author

Spotlight

Advertisements