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Issue 20, 2013
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A novel ratiometric fluorescence probe based on BSA assembled silver nanoclusters for mercuric ion selective sensing

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Abstract

A novel ratiometric fluorescence probe based on protein–silver nanoclusters (NCs) for mercuric ion (Hg2+) has been synthesized and characterized. A facile and simple one-step method for the synthesis of fluorescent Ag NCs, using bovine serum albumin (BSA) without preprocessing as a scaffold was developed. BSA assembled Ag NC (BSA-Ag NCs) were first found to exhibit ratiometric fluorescence with blue emission at 480 nm and red emission at 634 nm. Upon the addition of Hg2+, the emission of BSA-Ag NCs at 480 nm gradually increased and at 634 nm steadily decreased. The presence of Hg2+ induced a color change from light yellow to colorless and a bright red fluorescence to blue fluorescence. The determination of Hg2+ based on ratiometric fluorescence was developed. The linear dynamic range was from 5.0 × 10−8 M to 2.5 × 10−5 M with a detection limit of 4.87 × 10−8 M. The relative standard deviation (RSD) at 0.30 μM for Hg2+ of six replicates determination was 2.8%. The BSA-Ag NCs were conveniently and easily synthesized, so could serve as a “naked-eye” fluorescent probe for Hg2+. The probe may be a good candidate for the application of sensing Hg2+ in environmental analysis and fluorescence imaging.

Graphical abstract: A novel ratiometric fluorescence probe based on BSA assembled silver nanoclusters for mercuric ion selective sensing

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Publication details

The article was received on 28 May 2013, accepted on 05 Aug 2013 and first published on 07 Aug 2013


Article type: Paper
DOI: 10.1039/C3AY40901E
Citation: Anal. Methods, 2013,5, 5522-5527

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    A novel ratiometric fluorescence probe based on BSA assembled silver nanoclusters for mercuric ion selective sensing

    D. Lu, C. Zhang, L. Fan, H. Wu, S. Shuang and C. Dong, Anal. Methods, 2013, 5, 5522
    DOI: 10.1039/C3AY40901E

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