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Issue 5, 2013
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Quantification of ovarian cancer markers with integrated microfluidic concentration gradient and imaging nanohole surface plasmon resonance

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Abstract

Nanohole array-based biosensors integrated with a microfluidic concentration gradient generator were used for imaging detection and quantification of ovarian cancer markers. Calibration curves based on controlled concentrations of the analyte were created using a microfluidic stepped diffusive mixing scheme. Quantification of samples with unknown concentration of analyte was achieved by image-intensity comparison with the calibration curves. The biosensors were first used to detect the immobilization of ovarian cancer marker antibodies, and subsequently applied for the quantification of the ovarian cancer marker r-PAX8 (with a limit of detection of about 5 nM and a dynamic range from 0.25 to 9.0 μg.mL−1). The proposed biosensor demonstrated the ability of self-generating calibration curves on-chip in an integrated microfluidic platform, representing a further step towards the development of comprehensive lab-on-chip biomedical diagnostics based on nanohole array technology.

Graphical abstract: Quantification of ovarian cancer markers with integrated microfluidic concentration gradient and imaging nanohole surface plasmon resonance

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Article information


Submitted
05 Nov 2012
Accepted
09 Jan 2013
First published
10 Jan 2013

Analyst, 2013,138, 1450-1458
Article type
Paper

Quantification of ovarian cancer markers with integrated microfluidic concentration gradient and imaging nanohole surface plasmon resonance

C. Escobedo, Y. Chou, M. Rahman, X. Duan, R. Gordon, D. Sinton, A. G. Brolo and J. Ferreira, Analyst, 2013, 138, 1450
DOI: 10.1039/C3AN36616B

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