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Issue 20, 2013
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Convenient detection of E. coli in Ringer's solution

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Abstract

An easy and inexpensive detection method for DNA hybridization assays combining magnetic beads and enzymatically generated silver nanoparticles is introduced. The main advantage of this approach is the possibility to distinguish between positive and negative test results with the naked eye. In the case of complementary DNA sequences the sample will turn black within a few minutes, allowing readout without any hardware. In order to illustrate the applicability of the assay genomic DNA isolated from E. coli contaminated Ringer's solution was used for testing the sensitivity as well as specificity.

Graphical abstract: Convenient detection of E. coli in Ringer's solution

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Publication details

The article was received on 25 Jun 2013, accepted on 10 Aug 2013 and first published on 12 Aug 2013


Article type: Communication
DOI: 10.1039/C3AN01240A
Citation: Analyst, 2013,138, 5866-5870
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    Convenient detection of E. coli in Ringer's solution

    M. Schwarz, S. Pahlow, T. Bocklitz, C. Steinbrücker, D. Cialla, K. Weber and J. Popp, Analyst, 2013, 138, 5866
    DOI: 10.1039/C3AN01240A

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