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Issue 14, 2013
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Carbon nanotube decorated magnetic microspheres as an affinity matrix for biomolecules

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Abstract

Carbon nanotube (CNT) decorated magnetic microspheres were fabricated to develop a multimodal platform that utilizes non-covalent molecular interactions of CNTs to magnetically separate biomolecules. Hybrid CNT-microspheres prepared by a feasible method reported herein had a well-defined structure as characterized by Raman spectroscopy and scanning electron microscopy. Binding interactions of resulting magnetic CNT-microspheres with DNA oligonucleotides were studied to demonstrate that single stranded DNA (ssDNA) in a solution can be effectively recovered by magnetic CNT-microspheres through strong physical wrapping of DNA around CNTs' walls. The magnetic character of these CNT-microspheres combined with their capability to bind other molecules including DNA allows their use as an affinity matrix that can be utilized in affinity separation of biomolecules, and also as a platform to monitor non-covalent binding interactions of CNTs with other biomolecules. As a proof of concept, we report on the use of these CNT-microspheres in in vitro selection of ssDNA aptamers against carcinoembryonic antigen (CEA), a cancer biomarker, by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). ssDNA aptamer candidates that have strong affinity towards CEA were successfully separated magnetically from a pool of ssDNA (∼1014 molecules). Our results demonstrate that CNT-microspheres can serve as strong tools for affinity separation methodologies and can be utilized for various affinity pairs in solution.

Graphical abstract: Carbon nanotube decorated magnetic microspheres as an affinity matrix for biomolecules

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Publication details

The article was received on 21 Dec 2012, accepted on 04 Feb 2013 and first published on 05 Feb 2013


Article type: Paper
DOI: 10.1039/C3TB00563A
Citation: J. Mater. Chem. B, 2013,1, 1894-1902

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    Carbon nanotube decorated magnetic microspheres as an affinity matrix for biomolecules

    H. Ünal and J. H. Niazi, J. Mater. Chem. B, 2013, 1, 1894
    DOI: 10.1039/C3TB00563A

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