Jump to main content
Jump to site search

Issue 39, 2013
Previous Article Next Article

Hydrodynamic separation of proteins in supported lipid bilayers confined by gold barriers

Author affiliations

Abstract

Hydrodynamic drag forces generated by liquid flow above a supported lipid bilayer (SLB) can be used to induce lateral movement of molecules protruding from the SLB. Since the velocity of the individual molecules depends on their size and coupling to the lipid bilayer, these forces can also be used to enrich and separate different types of membrane-bound molecules. To improve and better quantify hydrodynamic-based molecular separation in SLBs, we formed the SLB on the floor of a microfluidic channel which was patterned with gold barriers that confined the lipid bilayer to a 100 μm wide strip in the center of a 300 μm wide microfluidic channel. This forces the SLB into a region of the channel where the spatial variation of the hydrodynamic forces is close to zero while at the same time preventing the SLB from creeping up on the PDMS sides of the channel, thus reducing the loss of material. We here use this approach to investigate the accumulation of (i) fluorescently labeled lipids and (ii) the protein complex cholera toxin B (CTB) and to compare how the accumulation and separation differ when having an infinite reservoir or only a spatially limited band of studied molecules in the SLB. In addition, we show how the method can be used for complete separation of different polyvalently bound fractions of CTB.

Graphical abstract: Hydrodynamic separation of proteins in supported lipid bilayers confined by gold barriers

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Jun 2013, accepted on 09 Aug 2013 and first published on 09 Aug 2013


Article type: Paper
DOI: 10.1039/C3SM51673C
Soft Matter, 2013,9, 9414-9419

  •   Request permissions

    Hydrodynamic separation of proteins in supported lipid bilayers confined by gold barriers

    B. Johansson, T. Olsson, P. Jönsson and F. Höök, Soft Matter, 2013, 9, 9414
    DOI: 10.1039/C3SM51673C

Search articles by author

Spotlight

Advertisements