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Issue 29, 2013
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Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

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Abstract

The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.

Graphical abstract: Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

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Article information


Submitted
11 Jan 2013
Accepted
27 Feb 2013
First published
07 Mar 2013

This article is Open Access

Soft Matter, 2013,9, 6752-6756
Article type
Communication

Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

S. Piluso, H. C. Cassell, J. L. Gibbons, T. E. Waller, N. J. Plant, A. F. Miller and G. Cavalli, Soft Matter, 2013, 9, 6752
DOI: 10.1039/C3SM00131H

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