Jump to main content
Jump to site search

Issue 10, 2013

Co-assemblies of micelle-forming diblock copolymers and enzymes on graphite substrate for an improved design of biosensor systems

Author affiliations

Abstract

The adsorption of ionic amphiphilic diblock copolymers comprising a polycationic block, polybutadiene-block-poly(2-(dimethylamino)ethyl methacrylate) (PB-b-PDMAEMA); and its quaternized derivative (PB-b-PDMAEMAq) from aqueous media onto graphite-based surfaces was examined. Both diblock copolymers in aqueous solution form star-like micelles with a hydrophobic PB core and a cationic corona built up from either strong cationic PDMAEMAq or pH-sensitive PDMAEMA. AFM experiments show that PB-b-PDMAEMAq micelles interact slightly with a graphite surface providing films with a low surface coverage. PB-b-PDMAEMA micelles adsorbed onto a graphite surface at pH ≥ 7 result in a more homogeneous coverage of the graphite surface by the diblock copolymer. The adsorption of two enzymes, tyrosinase (Tyr) and choline oxidase (ChO) on the graphite surface premodified with these diblock copolymers was also monitored by AFM and by electrochemical measurements of the enzymatic activities of PB-b-PDMAEMA–Tyr and PB-b-PDMAEMA–ChO films. A pronounced increase in the enzymatic activity of tyrosinase was observed with the increasing concentration of PB-b-PDMAEMA micelles in solution used for their depositions. Also, a pronounced increase in the enzymatic activities of both tyrosinase and choline oxidase was observed with the increasing pH of the deposition of the micelles from 2 to 10. The enzymatic activity increases with the coverage of the graphite surface with the preadsorbed copolymer. Finally, the polymer–enzyme films were tested as biosensors for phenol (when tyrosinase was adsorbed) and choline (when choline oxidase was adsorbed) and their activity and stability were compared to already existing setups.

Graphical abstract: Co-assemblies of micelle-forming diblock copolymers and enzymes on graphite substrate for an improved design of biosensor systems

Supplementary files

Article information


Submitted
05 Oct 2012
Accepted
03 Dec 2012
First published
25 Jan 2013

Soft Matter, 2013,9, 2858-2868
Article type
Paper

Co-assemblies of micelle-forming diblock copolymers and enzymes on graphite substrate for an improved design of biosensor systems

L. V. Sigolaeva, D. V. Pergushov, C. V. Synatschke, A. Wolf, I. Dewald, I. N. Kurochkin, A. Fery and A. H. E. Müller, Soft Matter, 2013, 9, 2858 DOI: 10.1039/C2SM27298A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements