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Issue 10, 2013
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Self-assembled light-driven photosynthetic-respiratory electron transport chain hybrid proton pump

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Abstract

One of the fundamental biochemical reactions providing the foundation of life is photosynthesis occurring in plants and cyanobacteria. Photon energy is converted into stored chemical energy which flows through various metabolic processes that in the case of most aerobic organisms, terminates in the electron transport chain. In this study we demonstrate a synthetic hybrid photosynthetic-respiratory chain based on ruthenium(II)-terpyridine linked to cytochrome c and coupled with cytochrome c oxidase. Upon irradiation at an initial pH of 7.2, we observe proton pumping at a rate of ≈3.3 × 103 H+ per s across the polymer bilayer generating a gradient up to ΔpH ≈ 0.2. Remarkably, cytochrome c oxidase appears to have a greater than 50% native mitochondrial orientation in the polymersome membrane. Our photoactive hybrid electron-proton chain generates a proton gradient that can store chemical energy which is a step towards the development of a model artificial protocell.

Graphical abstract: Self-assembled light-driven photosynthetic-respiratory electron transport chain hybrid proton pump

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Supplementary files

Article information


Submitted
26 Jun 2013
Accepted
18 Jul 2013
First published
19 Jul 2013

Chem. Sci., 2013,4, 3833-3838
Article type
Edge Article

Self-assembled light-driven photosynthetic-respiratory electron transport chain hybrid proton pump

D. Hvasanov, J. R. Peterson and P. Thordarson, Chem. Sci., 2013, 4, 3833
DOI: 10.1039/C3SC51780B

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