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Issue 10, 2013
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Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

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Abstract

The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4-dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150–180 s, three times longer than those without any treatments.

Graphical abstract: Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

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Publication details

The article was received on 22 Nov 2012, accepted on 10 Jan 2013 and first published on 11 Jan 2013


Article type: Communication
DOI: 10.1039/C3RA22999H
RSC Adv., 2013,3, 3237-3240

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    Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

    T. Yasui, K. Motoyama, N. Kaji, M. Tokeshi and Y. Baba, RSC Adv., 2013, 3, 3237
    DOI: 10.1039/C3RA22999H

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