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Issue 5, 2013
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Excitation energy migration and trapping on the surface of fluorescent poly(acrylic acid)-grafted polymer particles

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Abstract

The surface of poly(methyl methacrylate) particles with different amounts of a grafted layer of poly(acrylic acid) was labeled with varying degrees of an amino derivative of fluorescein isothiocyanate. The resulting fluorescent polymer particles were analyzed by absorption spectroscopy and by steady-state and time-resolved fluorescence spectroscopy including measurements of the fluorescence anisotropy. The combined results indicate that the overall decrease in fluorescence intensity with increasing surface concentrations of the fluorophore can be traced back to the formation of non-fluorescent aggregates. A mechanism is proposed, in which the excitation energy migrates between identical fluorophores until it is transferred to non-fluorescent aggregates acting as an energy trap. Increases in the surface fluorophore concentration increase both the probability for energy transfer between identical fluorophores and the probability for energy transfer to non-fluorescent aggregates. Furthermore, we suggest that this mechanism also applies to fluorescent protein conjugates and rationalizes the nonlinear dependence of the fluorescence emission on the labeling density.

Graphical abstract: Excitation energy migration and trapping on the surface of fluorescent poly(acrylic acid)-grafted polymer particles

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Article information


Submitted
01 Nov 2012
Accepted
21 Dec 2012
First published
21 Dec 2012

Photochem. Photobiol. Sci., 2013,12, 729-737
Article type
Paper

Excitation energy migration and trapping on the surface of fluorescent poly(acrylic acid)-grafted polymer particles

A. Hennig, S. Hatami, M. Spieles and U. Resch-Genger, Photochem. Photobiol. Sci., 2013, 12, 729
DOI: 10.1039/C2PP25364J

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