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Issue 11, 2013
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Rapid differentiation and quantification of live/dead cancer cells using differential photochemical behavior of acridine orange

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Abstract

This paper demonstrates for the first time a simple analytical method for differentiation and quantification of dead/live cancer cells using acridine orange (AO) enabled fluorescence spectroscopic techniques. Based on the differential fluorescence (live cells fluoresce green and dead cells orange) exhibited when intercalated with AO, the live/dead cells can be easily differentiated. The optimal AO concentration for enhanced sensitive differentiation has been optimized as 0.001%. These studies offer a promising application for rapid differentiation and quantification of live/dead cells in the case of cytotoxic treatment/therapies within several minutes.

Graphical abstract: Rapid differentiation and quantification of live/dead cancer cells using differential photochemical behavior of acridine orange

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Article information


Submitted
02 Jun 2013
Accepted
01 Aug 2013
First published
06 Aug 2013

Photochem. Photobiol. Sci., 2013,12, 1921-1926
Article type
Communication

Rapid differentiation and quantification of live/dead cancer cells using differential photochemical behavior of acridine orange

M. Manikandan and H. Wu, Photochem. Photobiol. Sci., 2013, 12, 1921
DOI: 10.1039/C3PP50165E

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