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Issue 1, 2013
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M1.3 – a small scaffold for DNA origami

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The DNA origami method produces programmable nanoscale objects that form when one long scaffold strand hybridizes to numerous oligonucleotide staple strands. One scaffold strand is dominating the field: M13mp18, a bacteriophage-derived vector 7249 nucleotides in length. The full-length M13 is typically folded by using over 200 staple oligonucleotides. Here we report the convenient preparation of a 704 nt fragment dubbed “M1.3” as a linear or cyclic scaffold and the assembly of small origami structures with just 15–24 staple strands. A typical M1.3 origami is large enough to be visualized by TEM, but small enough to show a cooperativity in its assembly and thermal denaturation that is reminiscent of oligonucleotide duplexes. Due to its medium size, M1.3 origami with globally modified staples is affordable. As a proof of principle, two origami structures with globally 5′-capped staples were prepared and were shown to give higher UV-melting points than the corresponding assembly with unmodified DNA. M1.3 has the size of a gene, not a genome, and may function as a model for gene-based nanostructures. Small origami with M1.3 as a scaffold may serve as a workbench for chemical, physical, and biological experiments.

Graphical abstract: M1.3 – a small scaffold for DNA origami

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The article was received on 21 Aug 2012, accepted on 26 Oct 2012 and first published on 30 Oct 2012

Article type: Paper
DOI: 10.1039/C2NR32393A
Nanoscale, 2013,5, 284-290

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    M1.3 – a small scaffold for DNA origami

    H. Said, V. J. Schüller, F. J. Eber, C. Wege, T. Liedl and C. Richert, Nanoscale, 2013, 5, 284
    DOI: 10.1039/C2NR32393A

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