Jump to main content
Jump to site search

Issue 6, 2013
Previous Article Next Article

Detection of high molecular weight proteins by MALDI imaging mass spectrometry

Author affiliations

Abstract

MALDI imaging mass spectrometry (IMS) is a unique technology to explore the spatial distribution of biomolecules directly on tissues. It allows the in situ investigation of a large number of small proteins and peptides. Detection of high molecular weight proteins through MALDI IMS still represents an important challenge, as it would allow the direct investigation of the distribution of more proteins involved in biological processes, such as cytokines, enzymes, neuropeptide precursors and receptors. In this work we compare the traditional method performed with sinapinic acid with a comparable protocol using ferulic acid as the matrix. Data show a remarkable increase of signal acquisition in the mass range of 20k to 150k Th. Moreover, we report molecular images of biomolecules above 70k Th, demonstrating the possibility of expanding the application of this technology both in clinical investigations and basic science.

Graphical abstract: Detection of high molecular weight proteins by MALDI imaging mass spectrometry

Back to tab navigation

Supplementary files

Article information


Submitted
25 Jul 2012
Accepted
19 Dec 2012
First published
20 Dec 2012

Mol. BioSyst., 2013,9, 1101-1107
Article type
Method

Detection of high molecular weight proteins by MALDI imaging mass spectrometry

V. Mainini, G. Bovo, C. Chinello, E. Gianazza, M. Grasso, G. Cattoretti and F. Magni, Mol. BioSyst., 2013, 9, 1101
DOI: 10.1039/C2MB25296A

Search articles by author

Spotlight

Advertisements