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Issue 11, 2013
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Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection

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Abstract

A microfluidic device that enables on-chip matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) detection for liquid chromatography (LC) separations is described. The device comprises an array of functional elements to carry out LC separations, integrates a novel microchip-MS interface to facilitate the orthogonal transposition of the microfluidic LC channel into an array of reservoirs, and enables sensitive MALDI-MS detection directly from the chip. Essentially, the device provides a snapshot MALDI-MS map of the content of the separation channel present on the chip. The detection of proteins with biomarker potential from MCF10A breast epithelial cell extracts, and detection limits in the low fmol range, are demonstrated. In addition, the design of the novel LC-MALDI-MS chip entices the promotion of a new concept for performing sample separations within the limited time-frame that accompanies the dead-volume of a separation channel.

Graphical abstract: Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection

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Publication details

The article was received on 10 Feb 2013, accepted on 20 Mar 2013 and first published on 20 Mar 2013


Article type: Paper
DOI: 10.1039/C3LC50190F
Citation: Lab Chip, 2013,13, 2055-2065
  • Open access: Creative Commons BY-NC license
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    Microfluidic LC device with orthogonal sample extraction for on-chip MALDI-MS detection

    I. M. Lazar and J. L. Kabulski, Lab Chip, 2013, 13, 2055
    DOI: 10.1039/C3LC50190F

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