Jump to main content
Jump to site search

Issue 6, 2013
Previous Article Next Article

Microfluidics study of intracellular calcium response to mechanical stimulation on single suspension cells

Author affiliations

Abstract

A microfluidic microdevice was developed to exert mechanical stimulation on an individual suspension cell for mechanosensation research. In this microfluidic chip, an individual cell was isolated from a population of cells, and trapped in a microchannel with a compressive component made of a deflectable membrane. The mechanosensation of HL60 cells (leukemic cells) was studied using this chip, and the results showed that mechanical stimulations could trigger extracellular calcium to flow into HL60 cells through ion channels on cell membranes. The tension on individual HL60 cells exerted by the microdevice was showed large variations in the threshold of mechanosensation activation. In contrast to previous reports using patch clamp technique, there was little influence of cytoskeleton interruption on HL60 cell mechanosensation triggered by whole-cell compression. Additionally, two functional units were integrated in one chip for carrying out mechanosensation study in parallel, where HL60 cells (leukemic cells) and Jurkat cells (lymphocytes) were shown to respond to mechanical stimulation with different kinetics. The results demonstrated that the microfluidic device provides a novel approach to investigating the mechanosensation of single suspension cells in high-throughput.

Graphical abstract: Microfluidics study of intracellular calcium response to mechanical stimulation on single suspension cells

Back to tab navigation

Publication details

The article was received on 02 Aug 2012, accepted on 17 Jan 2013 and first published on 18 Jan 2013


Article type: Paper
DOI: 10.1039/C3LC40880A
Lab Chip, 2013,13, 1060-1069

  •   Request permissions

    Microfluidics study of intracellular calcium response to mechanical stimulation on single suspension cells

    T. Xu, W. Yue, C. Li, X. Yao and M. Yang, Lab Chip, 2013, 13, 1060
    DOI: 10.1039/C3LC40880A

Search articles by author

Spotlight

Advertisements