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Issue 2, 2013
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Visualizing viral assemblies in a nanoscale biosphere

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Abstract

We present a novel microfluidic platform to examine biological assemblies at high-resolution. We have engineered a functionalized chamber that serves as a “nanoscale biosphere” to capture and maintain rotavirus double-layered particles (DLPs) in a liquid environment. The chamber can be inserted into the column of a transmission electron microscope while being completely isolated from the vacuum system. This configuration allowed us to determine the structure of biological complexes at nanometer-resolution within a self-contained vessel. Images of DLPs were used to calculate the first 3D view of macromolecules in solution. We refer to this new fluidic visualization technology as in situ molecular microscopy.

Graphical abstract: Visualizing viral assemblies in a nanoscale biosphere

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Supplementary files

Article information


Submitted
15 Jun 2012
Accepted
13 Nov 2012
First published
19 Nov 2012

This article is Open Access

Lab Chip, 2013,13, 216-219
Article type
Technical Innovation

Visualizing viral assemblies in a nanoscale biosphere

B. L. Gilmore, S. P. Showalter, M. J. Dukes, J. R. Tanner, A. C. Demmert, S. M. McDonald and D. F. Kelly, Lab Chip, 2013, 13, 216
DOI: 10.1039/C2LC41008G

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