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Issue 24, 2013
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DNA sequence analysis with droplet-based microfluidics

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Abstract

Droplet-based microfluidic techniques can form and process micrometer scale droplets at thousands per second. Each droplet can house an individual biochemical reaction, allowing millions of reactions to be performed in minutes with small amounts of total reagent. This versatile approach has been used for engineering enzymes, quantifying concentrations of DNA in solution, and screening protein crystallization conditions. Here, we use it to read the sequences of DNA molecules with a FRET-based assay. Using probes of different sequences, we interrogate a target DNA molecule for polymorphisms. With a larger probe set, additional polymorphisms can be interrogated as well as targets of arbitrary sequence.

Graphical abstract: DNA sequence analysis with droplet-based microfluidics

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Publication details

The article was received on 03 Aug 2013, accepted on 04 Oct 2013 and first published on 04 Nov 2013


Article type: Paper
DOI: 10.1039/C3LC50905B
Lab Chip, 2013,13, 4864-4869

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    DNA sequence analysis with droplet-based microfluidics

    A. R. Abate, T. Hung, R. A. Sperling, P. Mary, A. Rotem, J. J. Agresti, M. A. Weiner and D. A. Weitz, Lab Chip, 2013, 13, 4864
    DOI: 10.1039/C3LC50905B

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