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Issue 22, 2013
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Parallel measurements of reaction kinetics using ultralow-volumes

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Abstract

We present a new platform for the production and manipulation of microfluidic droplets in view of measuring the evolution of a chemical reaction. Contrary to existing approaches, our device uses gradients of confinement to produce a single drop on demand and guide it to a pre-determined location. In this way, two nanoliter drops containing different reagents can be placed in contact and merged together, in order to trigger a chemical reaction. The reaction rate is extracted from an analysis of the observed reaction–diffusion front. We show that the results obtained using this platform are in excellent agreement with stopped-flow measurements, while decreasing the sample consumption 5000 fold. We also show how the device operation can be parallelized in order to react an initial sample with a range of compounds or concentrations, on a single integrated chip. This integrated chip thus further reduces sample consumption while reducing the time required for the experimental runs from hours to minutes.

Graphical abstract: Parallel measurements of reaction kinetics using ultralow-volumes

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Publication details

The article was received on 26 Jun 2013, accepted on 28 Aug 2013 and first published on 29 Aug 2013


Article type: Paper
DOI: 10.1039/C3LC50768H
Lab Chip, 2013,13, 4326-4330

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    Parallel measurements of reaction kinetics using ultralow-volumes

    E. Fradet, P. Abbyad, M. H. Vos and C. N. Baroud, Lab Chip, 2013, 13, 4326
    DOI: 10.1039/C3LC50768H

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