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Issue 9, 2013
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Universally applicable three-dimensional hydrodynamic microfluidic flow focusing

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Abstract

We have demonstrated a microfluidic device that can not only achieve three-dimensional flow focusing but also confine particles to the center stream along the channel. The device has a sample channel of smaller height and two sheath flow channels of greater height, merged into the downstream main channel where 3D focusing effects occur. We have demonstrated that both beads and cells in our device display significantly lower CVs in velocity and position distributions as well as reduced probability of coincidental events than they do in conventional 2D-confined microfluidic channels. The improved particle confinement in the microfluidic channel is highly desirable for microfluidic flow cytometers and in fluorescence-activated cell sorting (FACS). We have also reported a novel method to measure the velocity of each individual particle in the microfluidic channel. The method is compatible with the flow cytometer setup and requires no sophisticated visualization equipment. The principles and methods of device design and characterization can be applicable to many types of microfluidic systems.

Graphical abstract: Universally applicable three-dimensional hydrodynamic microfluidic flow focusing

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Article information


Submitted
30 Oct 2012
Accepted
21 Feb 2013
First published
22 Feb 2013

This article is Open Access

Lab Chip, 2013,13, 1803-1809
Article type
Paper

Universally applicable three-dimensional hydrodynamic microfluidic flow focusing

Y. Chiu, S. H. Cho, Z. Mei, V. Lien, T. Wu and Y. Lo, Lab Chip, 2013, 13, 1803
DOI: 10.1039/C3LC41202D

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