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Issue 7, 2013
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Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients

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Abstract

An automated microfluidic cell culture platform that creates and maintains independently programmable diffusion-based gradients is reported. Temporal modulation of the source and sink flow patterns allow generation of extremely stable spatial gradients. We developed a system that integrates 30 parallel gradients in a single device, with 10 different chemical formulations and 3 replicates. Mammalian fibroblast and macrophage cells were screened for NFκB pathway activity under gradients of TNFα, PDGF, and LPS, and multiparameter measurements were performed to demonstrate the capability of the device in dynamic single-cell analysis.

Graphical abstract: Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients

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Submitted
23 Sep 2012
Accepted
17 Jan 2013
First published
18 Jan 2013

Lab Chip, 2013,13, 1273-1281
Article type
Paper

Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients

T. Frank and S. Tay, Lab Chip, 2013, 13, 1273
DOI: 10.1039/C3LC41076E

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