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Issue 1, 2013
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Passive circulating cell sorting by deformability using a microfluidic gradual filter

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Abstract

The deformability of circulating leukocytes plays an important role in the physiopathology of several diseases like sepsis or acute respiratory distress syndrome (ARDS). We present here a microfluidic method for the passive testing, sorting and separating of non-adherent cell populations by deformability. It consists of microfluidic sieves in series with pore sizes decreasing from the upstream to the downstream. The method capabilities are demonstrated with monocytic cell lines (THP-1) treated by Jasplakinolide (a stabilizer of polymerized actin), LatrunculinA (an inhibitor of actin polymerization), and with the plasma of patients suffering from ARDS. Simple sample injection with standard syringes and pumps makes the method readily adapted for experimentation in hospitals.

Graphical abstract: Passive circulating cell sorting by deformability using a microfluidic gradual filter

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Article information


Submitted
25 Jul 2012
Accepted
16 Oct 2012
First published
17 Oct 2012

Lab Chip, 2013,13, 161-170
Article type
Paper

Passive circulating cell sorting by deformability using a microfluidic gradual filter

P. Preira, V. Grandné, J.-M. Forel, S. Gabriele, M. Camara and O. Theodoly, Lab Chip, 2013, 13, 161
DOI: 10.1039/C2LC40847C

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