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Issue 26, 2013
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A highly sensitive luminescent lectin sensor based on an α-D-mannose substituted Tb3+ antenna complex

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Abstract

Lectin–carbohydrate interactions are the basis of many biological processes and essentially they constitute the language through which intercellular communications are codified. Thus they represent powerful tools in the examination and interpretation of changes that occur on cell surfaces during both physiological and, more importantly, pathological events. The development of optical techniques that exploit the unique properties of luminescent lanthanoid metal complexes in the investigation of lectin–carbohydrate recognition can foster research in the field of ratiometric biosensing and disease detection. Here we report the synthesis of a Tb3+-DO3A complex (Tb⊂1) bearing an α-D-mannose residue and the related study of binding affinity with concanavalin A (Con A) labeled with rhodamine-B-isothiocyanate (RITC-Con A). Luminescence spectroscopy and dynamic studies show changes in emission spectra that can be ascribed to a luminescence resonance energy transfer (LRET) from Tb⊂1 (donor) to RITC-Con A (acceptor). The binding constant value between the two species was found to be one order of magnitude larger than those previously reported for similar types of recognition. To the best of our knowledge this is the first example of the use of a pre-organized luminescent lanthanoid complex in the study of carbohydrate–protein interactions by LRET.

Graphical abstract: A highly sensitive luminescent lectin sensor based on an α-d-mannose substituted Tb3+ antenna complex

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Publication details

The article was received on 17 Dec 2012, accepted on 14 Feb 2013 and first published on 14 Feb 2013


Article type: Paper
DOI: 10.1039/C3DT33023K
Dalton Trans., 2013,42, 9453-9461

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    A highly sensitive luminescent lectin sensor based on an α-D-mannose substituted Tb3+ antenna complex

    E. Martín Rodríguez, N. Bogdan, J. A. Capobianco, S. Orlandi, M. Cavazzini, C. Scalera and S. Quici, Dalton Trans., 2013, 42, 9453
    DOI: 10.1039/C3DT33023K

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