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Issue 12, 2013
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Formation of lipase Candida sp. 99–125 CLEAs in mesoporous silica: characterization and catalytic properties

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Abstract

Mesoporous silica (MPS) was synthesized and used as a support for lipase Candida sp. 99–125 immobilization. The immobilization procedure was simple and effective: lipase Candida sp. 99–125 was first immobilized in the MPS by adsorption (named ADL@MPS), then chemical crosslinking was conducted for stabilizing the lipase and inhibiting leakage, and cross-linked enzyme aggregates (CLEAs) of Candida sp. 99–125 lipase in the MPS were obtained (named CLL@MPS). The stability of ADL@MPS and CLL@MPS was investigated. Compared with ADL@MPS and native lipase, CLL@MPS showed outstanding stability under vigorous shaking conditions and the thermal stability of CLL@MPS in the presence of organic solvents was also improved. Additionally, CLL@MPS exhibited high catalytic performance in hydrolysis, esterification, and transesterification reactions with increased stability and recyclability.

Graphical abstract: Formation of lipase Candida sp. 99–125 CLEAs in mesoporous silica: characterization and catalytic properties

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Publication details

The article was received on 16 Jun 2013, accepted on 24 Sep 2013 and first published on 25 Sep 2013


Article type: Paper
DOI: 10.1039/C3CY00412K
Catal. Sci. Technol., 2013,3, 3353-3359

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    Formation of lipase Candida sp. 99–125 CLEAs in mesoporous silica: characterization and catalytic properties

    J. Gao, L. Shi, Y. Jiang, L. Zhou and Y. He, Catal. Sci. Technol., 2013, 3, 3353
    DOI: 10.1039/C3CY00412K

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