Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 22nd May 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 22, 2013
Previous Article Next Article

Proteotyping for the rapid identification of influenza virus and other biopathogens

Author affiliations

Abstract

The influenza virus is one of the most deadly infectious agents known to man and has been responsible for the deaths of some hundred million lives throughout human history. The need to rapidly and reliably survey circulating virus strains down to the molecular level is ever present. This tutorial describes the development and application of a new proteotyping approach that harnesses the power of high resolution of mass spectrometry to characterise the influenza virus, and by extension other bacterial and viral pathogens. The approach is shown to be able to type, subtype, and determine the lineage of human influenza virus strains through the detection of one or more signature peptide ions in the mass spectrum of whole virus digests. Pandemic strains can be similarly distinguished from seasonal ones, and new computer algorithms have been written to allow reassorted strains that pose the greatest pandemic risk to be rapidly identified from such datasets. The broader application of the approach is further demonstrated here for the parainfluenza virus, a virus which can be life threatening to children and presents similar clinical symptoms to influenza.

Graphical abstract: Proteotyping for the rapid identification of influenza virus and other biopathogens

Back to tab navigation

Publication details

The article was received on 28 Feb 2013 and first published on 30 Apr 2013


Article type: Tutorial Review
DOI: 10.1039/C3CS60081E
Chem. Soc. Rev., 2013,42, 8584-8595

  •   Request permissions

    Proteotyping for the rapid identification of influenza virus and other biopathogens

    K. M. Downard, Chem. Soc. Rev., 2013, 42, 8584
    DOI: 10.1039/C3CS60081E

Search articles by author

Spotlight

Advertisements