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Issue 39, 2013
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Light-induced reactivation of O2-tolerant membrane-bound [Ni–Fe] hydrogenase from the hyperthermophilic bacterium Aquifex aeolicus under turnover conditions

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Abstract

We report the effect of UV-Vis light on the membrane-bound [Ni–Fe] hydrogenase from Aquifex aeolicus under turnover conditions. Using electrochemistry, we show a potential dependent light sensitivity and propose that a light-induced structural change of the [Ni–Fe] active site is related to an enhanced reactivation of the hydrogenase under illumination at high potentials.

Graphical abstract: Light-induced reactivation of O2-tolerant membrane-bound [Ni–Fe] hydrogenase from the hyperthermophilic bacterium Aquifex aeolicus under turnover conditions

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Article information


Submitted
21 Jun 2013
Accepted
12 Aug 2013
First published
19 Aug 2013

Phys. Chem. Chem. Phys., 2013,15, 16463-16467
Article type
Communication

Light-induced reactivation of O2-tolerant membrane-bound [Ni–Fe] hydrogenase from the hyperthermophilic bacterium Aquifex aeolicus under turnover conditions

A. Ciaccafava, C. Hamon, P. Infossi, V. Marchi, M. Giudici-Orticoni and E. Lojou, Phys. Chem. Chem. Phys., 2013, 15, 16463
DOI: 10.1039/C3CP52596A

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