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Issue 96, 2013
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Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG)

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Abstract

The visualization of Golgi glycosylation defects in patients' cells with Congenital Disorders of Glycosylation (CDG) is challenging and necessitates the use of cumbersome glycan analysis methods that are barely adapted to clinical research. We show here that metabolic labelling of patient cells with alkyne-tagged sialic-acid (SiaNAl) enables an easy and reliable readout assay for the detection of CDG occurrence. It also provides valuable clues regarding the pathological processes by assessing the distribution of sialic acid analogues within the cells.

Graphical abstract: Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG)

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Article information


Submitted
02 Aug 2013
Accepted
05 Oct 2013
First published
09 Oct 2013

Chem. Commun., 2013,49, 11293-11295
Article type
Communication

Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG)

J. Vanbeselaere, D. Vicogne, G. Matthijs, C. Biot, F. Foulquier and Y. Guerardel, Chem. Commun., 2013, 49, 11293
DOI: 10.1039/C3CC45914D

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