Issue 66, 2013

Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention

Abstract

The powerful strategy of “intracellular click reaction” was used to retain a chemical Ca2+ indicator in the cytosol. Specifically, a novel clickable Ca2+ indicator “N3-fura-2 AM” was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca2+ oscillation was observed for an extended period of time.

Graphical abstract: Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention

Supplementary files

Article information

Article type
Communication
Submitted
06 Apr 2013
Accepted
16 May 2013
First published
16 May 2013

Chem. Commun., 2013,49, 7313-7315

Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention

Y. Takei, A. Murata, K. Yamagishi, S. Arai, H. Nakamura, T. Inoue and S. Takeoka, Chem. Commun., 2013, 49, 7313 DOI: 10.1039/C3CC42489H

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