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Issue 49, 2013
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A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

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Abstract

A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied to the evaluation of protein kinase inhibitors.

Graphical abstract: A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

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Publication details

The article was received on 06 Mar 2013, accepted on 29 Apr 2013 and first published on 30 Apr 2013


Article type: Communication
DOI: 10.1039/C3CC41680A
Chem. Commun., 2013,49, 5592-5594

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    A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

    S. Shiosaki, T. Nobori, T. Mori, R. Toita, Y. Nakamura, C. W. Kim, T. Yamamoto, T. Niidome and Y. Katayama, Chem. Commun., 2013, 49, 5592
    DOI: 10.1039/C3CC41680A

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