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A modular approach to obtain benzimidazolium–urea-based, fluorophore-appended macrocyclic receptors was developed. This class of receptors could be used as selective ratiometric fluorescent sensors for H2PO4 due to the synergistic binding effect of benzimidazolium and urea moieties.

Graphical abstract: Novel benzimidazolium–urea-based macrocyclic fluorescent sensors: synthesis, ratiometric sensing of H2PO4− and improvement of the anion binding performance via a synergistic binding strategy

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