Issue 6, 2013

The impact of mass spectrometry on the study of intact antibodies: from post-translational modifications to structural analysis

Abstract

Monoclonal antibodies (mAbs) are important therapeutics, targeting a variety of diseases ranging from cancers to neurodegenerative disorders. In developmental stages and prior to clinical use, these molecules require thorough structural characterisation, but their large size and heterogeneity present challenges for most analytical techniques. Over the past 20 years, mass spectrometry (MS) has transformed from a tool for small molecule analysis to a technique that can be used to study large intact proteins and non-covalent protein complexes. Here, we review several MS-based techniques that have emerged for the analysis of intact mAbs and discuss the prospects of using these technologies for the analysis of biopharmaceuticals.

Graphical abstract: The impact of mass spectrometry on the study of intact antibodies: from post-translational modifications to structural analysis

Article information

Article type
Feature Article
Submitted
17 Sep 2012
Accepted
14 Nov 2012
First published
15 Nov 2012

Chem. Commun., 2013,49, 538-548

The impact of mass spectrometry on the study of intact antibodies: from post-translational modifications to structural analysis

N. J. Thompson, S. Rosati, R. J. Rose and A. J. R. Heck, Chem. Commun., 2013, 49, 538 DOI: 10.1039/C2CC36755F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements