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Issue 9, 2013
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Traceless protein delivery with an efficient recyclable nanocarrier

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Abstract

Intracellular delivery is a prerequisite for the efficacy of many pharmaceutical proteins. Herein, vitamin B6 (pyridoxal-5′-phosphate, PLP) functionalized calcium phosphate (CP) is used as the bio-recyclable nanocarrier for delivery of proteins into cells. Proteins could be loaded on/released from PLP-CP via formation/hydrolysis of pH sensitive aldimine bridging lysine and surface-displayed PLP. The loaded proteins could be delivered into the cytosol of HeLa, HepG2 and L929 cells where the carrier could be metabolized into endogenous metabolites of Ca2+, HPO42−, and vitamin B6. PLP-CP mediated cell transduction is 10–40 folds more efficient than TAT which is a widely used cell penetrating peptide, demonstrating the utility of PLP-CP as the traceless platform for high-efficiency delivery of proteins into mammalian cells.

Graphical abstract: Traceless protein delivery with an efficient recyclable nanocarrier

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Article information


Submitted
14 May 2013
Accepted
06 Jun 2013
First published
03 Jul 2013

Biomater. Sci., 2013,1, 918-923
Article type
Paper

Traceless protein delivery with an efficient recyclable nanocarrier

X. Wu, Y. Song, J. Han, L. Yang and S. Han, Biomater. Sci., 2013, 1, 918
DOI: 10.1039/C3BM60128E

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