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Issue 7, 2013
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Qualitative and quantitative analysis in solid-state fermentation of protein feed by FT-NIR spectroscopy integrated with multivariate data analysis

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Abstract

The potential of Fourier-transform near-infrared (FT-NIR) spectroscopy for qualitative and quantitative analysis in solid-state fermentation (SSF) of protein feed was verified based on FT-NIR spectroscopy combined with multivariate data analysis. The raw spectra were processed and analyzed by multivariate analyses, which integrated the approaches of discrete wavelet transform (DWT), principal component analysis and extreme learning machine (ELM) modeling. The noise of raw spectra was filtered and latent information was extracted by DWT, and then the characteristic information obtained by DWT was visualized in principal component space, in which the structures with the time course of the SSF were explored. Thereafter, some parameters of the calibration models were optimized by cross-validation. The results of the final models were achieved as follows: root mean square error of prediction (RMSEP) = 0.0987/Rp2 = 0.9322 for pH model, RMSEP = 0.0092 w/w/Rp2 = 0.8991 for moisture content model, and an identification rate of 91.43% for the discrimination model of the fermentation phase in the validation set. Finally, compared with partial least squares (PLS)/PLS-discriminant analysis and back propagation artificial neural network models, the ELM model showed excellent performance for prediction and generalization. This study demonstrates that FT-NIR spectroscopy coupled with appropriate chemometrics approaches could be utilized to monitor the SSF, and ELM reveals its superiority in model calibration.

Graphical abstract: Qualitative and quantitative analysis in solid-state fermentation of protein feed by FT-NIR spectroscopy integrated with multivariate data analysis

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Publication details

The article was received on 23 Oct 2012, accepted on 13 Feb 2013 and first published on 13 Feb 2013


Article type: Paper
DOI: 10.1039/C3AY26601J
Anal. Methods, 2013,5, 1872-1880

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    Qualitative and quantitative analysis in solid-state fermentation of protein feed by FT-NIR spectroscopy integrated with multivariate data analysis

    H. Jiang, G. Liu, C. Mei and Q. Chen, Anal. Methods, 2013, 5, 1872
    DOI: 10.1039/C3AY26601J

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