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Issue 7, 2013
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Monitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatography

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Abstract

Nux-vomica is a tropical plant that contains two ergot alkaloids i.e. strychnine and brucine which have pharmaceutical and toxic properties. In some places, for example in India, Nux-vomica is used in ayurvedic preparations in homeopathic medicine. As both compounds are toxic monitoring is required. In this work a method for the chromatographic determination of strychnine and brucine in various herbal/ayurvedic sample preparations, homeopathic medicines, containing Nux-vomica, seeds of Nux-vomica as well as in spiked serum and real urine samples are described. The method involves a direct injection micellar liquid chromatography technique that was optimised under ICH guidelines. Optimum conditions are a mobile phase composed by 0.10 M SDS-pentanol 4% (v/v)-NaH2PO4 buffered at pH 3 with UV detection at 258 nm, flow rate of 1 mL min−1, and temperature 25 °C. Under these conditions strychnine and brucine were analyzed in 15 and 11 min, respectively. Validation studies using ICH guidelines were performed to show that the proposed method was linear (r2 > 0.9997), with LODs (0.18 and 0.09) and LOQs (0.69 and 0.35), for strycnine and brucine, respectively. Repeatability intra- and inter-day was below of 2.50. The developed method can be useful for analyzing strychnine and brucine for quality control and other related forensic and clinical cases.

Graphical abstract: Monitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatography

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Publication details

The article was received on 08 Sep 2012, accepted on 11 Jan 2013 and first published on 14 Jan 2013


Article type: Paper
DOI: 10.1039/C3AY26014C
Anal. Methods, 2013,5, 1747-1754

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    Monitoring strychnine and brucine in biochemical samples using direct injection micellar liquid chromatography

    S. D. Mishra, D. Bose, S. K. Shukla, A. Durgabanshi and J. Esteve-Romero, Anal. Methods, 2013, 5, 1747
    DOI: 10.1039/C3AY26014C

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