Jump to main content
Jump to site search

Issue 21, 2013
Previous Article Next Article

Comparative LC-MS/MS analysis of optimal cutting temperature (OCT) compound removal for the study of mammalian proteomes

Author affiliations

Abstract

Primary tissue samples are valuable resources for investigators interested in understanding disease. In order to maximize the information content that can be gained from these precious samples, proper storage, handling, and preparation are essential. Some tissue preservation techniques utilize the cryopreservation medium, optimal cutting temperature (OCT) compound. While this medium provides benefits for traditional molecular studies, certain components can interfere with mass spectrometric analyses. Mass spectrometry based proteomics is a growing field with many applications for disease research. Our goal is to determine a reliable method for separating the proteins from the contaminating species in OCT embedded samples, thus making these samples compatible with mass spectrometric analyses. The novel applications of ether–methanol precipitation, filter-aided sample preparation (FASP), and SDS-PAGE provide researchers with protocols for removing OCT contaminating species from valuable samples. The results presented in this study show that all three methods reproducibly remove OCT; however, precipitation and FASP outperform SDS-PAGE by common proteomic metrics.

Graphical abstract: Comparative LC-MS/MS analysis of optimal cutting temperature (OCT) compound removal for the study of mammalian proteomes

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Jun 2013, accepted on 10 Sep 2013 and first published on 13 Sep 2013


Article type: Paper
DOI: 10.1039/C3AN01121F
Analyst, 2013,138, 6380-6384

  •   Request permissions

    Comparative LC-MS/MS analysis of optimal cutting temperature (OCT) compound removal for the study of mammalian proteomes

    L. A. Weston and A. B. Hummon, Analyst, 2013, 138, 6380
    DOI: 10.1039/C3AN01121F

Search articles by author

Spotlight

Advertisements