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Issue 6, 2013
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Antibody orientation on biosensor surfaces: a minireview

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Abstract

Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains – the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.

Graphical abstract: Antibody orientation on biosensor surfaces: a minireview

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Article information


Submitted
01 Dec 2012
Accepted
06 Jan 2013
First published
22 Jan 2013

This article is Open Access

Analyst, 2013,138, 1619-1627
Article type
Minireview

Antibody orientation on biosensor surfaces: a minireview

A. K. Trilling, J. Beekwilder and H. Zuilhof, Analyst, 2013, 138, 1619
DOI: 10.1039/C2AN36787D

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    [Original citation] - Published by The Royal Society of Chemistry.

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