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Issue 7, 2013
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Label-free, disposable fiber-optic biosensors for DNA hybridization detection

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Abstract

A novel and highly sensitive fiber-optic DNA sensor based on a thin-core fiber modal interferometer (TCFMI) is demonstrated by using a layer-by-layer (LbL) self-assembly technology. Poly(ethylenimine) (PEI), poly(acrylic acid) (PAA) and single-stranded DNA (ssDNA) were used for the preparation of a polyelectrolyte multilayer film for DNA detection. The film thickness was measured through a surface profilometer. The surface morphologies of (PEI/PAA)4, (PEI/PAA)4.5 and (PEI/PAA)4(PEI/DNA)1 multilayer films were characterized by atomic force microscopy. The fabricated DNA sensors were tested with different types of target ssDNA solutions with a concentration of 1 μM. The results show that the sensitivity of the TCFMI-based ssDNA sensor is 0.27 nm/matched-base at the concentration of 1 μM and can even distinguish the number of matched bases of ssDNA chains.

Graphical abstract: Label-free, disposable fiber-optic biosensors for DNA hybridization detection

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Submitted
24 Aug 2012
Accepted
15 Jan 2013
First published
16 Jan 2013

Analyst, 2013,138, 1988-1994
Article type
Paper

Label-free, disposable fiber-optic biosensors for DNA hybridization detection

M. Yin, C. Wu, L. Shao, W. K. E. Chan, A. P. Zhang, C. Lu and H. Tam, Analyst, 2013, 138, 1988
DOI: 10.1039/C3AN36791F

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