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Issue 6, 2013
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Biomolecular analysis and cancer diagnostics by negative mode probe electrospray ionization

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Abstract

We have examined several combinations of solvents and probes with the aim of optimizing the ionization conditions for biomolecules e.g., proteins, peptides and lipids by negative mode probe electrospray ionization mass spectrometry (PESI-MS). With the data presented in this study, negative-mode PESI-MS can be considered as a potential tool for biomolecular analysis and cancer diagnostics because of its simplicity in instrumental configuration. A sharper sampling probe was found to be better for obtaining high quality mass spectra because it can generate stable electrospray without the occurrence of gas breakdown. Although the best conditions may depend on each sample, aqueous organic solvent solutions, especially isopropanol–H2O (1/1) with a pH of ≥7, are shown to be preferable for negative-mode PESI-MS, which was successfully applied to colon cancer diagnosis.

Graphical abstract: Biomolecular analysis and cancer diagnostics by negative mode probe electrospray ionization

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Supplementary files

Article information


Submitted
25 Oct 2012
Accepted
09 Jan 2013
First published
10 Jan 2013

Analyst, 2013,138, 1682-1688
Article type
Paper

Biomolecular analysis and cancer diagnostics by negative mode probe electrospray ionization

M. K. Mandal, S. Saha, K. Yoshimura, Y. Shida, S. Takeda, H. Nonami and K. Hiraoka, Analyst, 2013, 138, 1682
DOI: 10.1039/C3AN36554A

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